In a field experiment conducted in 1998-2002 on sandy-loam soil the effect of applying chosen growth regulators in an American ginseng culture was examined. In the second year of vegetation (June 2000) plants were sprayed with following substances (each in a dose of 100 and 200 mg/l): kinetin, daminozide, mixture (1:1) of gibberellic acid (GA3) with potassium salt of alfa-naphtyl acetic acid - KNAA and IPO-1 (benzimidazole derivative) - a new preparation obtained from Institute of Organic Industry in Warsaw. Amongst the applied growth regulators positive effect on growth of plants and yield of roots, as well as on the content of active compounds had IPO-1, daminozide (in a dose of 100-200 mg/l) and, to a certain extent, kinetin (200 mg/l), while the mixture of GA3 and KNAA affected the above-mentioned features negatively.