The essential oils isolated from linalool (L), geranial/geraniol/neral (G/G/N) and thymol (T) chemotypes of Thymus pulegioides L. were investigated for antifungal properties using pathogenic fungi of Candida species. Tested chemotypes showed different fungicidal activities. The Candida genus was most significantly affected by essential oils of T chemotype and most weakly affected by essential oil of L chemotype. The G/G/N chemotype showed the strongest effect on C. albicans CA1 and the weakest on C. parapsilosis CP1. Assessment of viability of yeast cells showed that cell viability after 60 min. of incubation with different chemotypes decreased 5 times in comparison with control.