Effect of Willow herb (Epilobium angustifolium L.) extract on gene expression of selected P450 cytochromes in rat liver – preliminary study

RADOSŁAW KUJAWSKI, MARCIN OŻAROWSKI, NATALIA DEREBECKA - HOŁYSZ, JOANA BARTKOWIAK - WIECZOREK, ANNA BOGACZ, MONIKA KARASIEWICZ, PRZEMYSŁAW Ł. MIKOŁAJCZAK, TERESA BOBKIEWICZ - KOZŁOWSKA, PRZEMYSŁAW M. MROZIKIEWICZ

Abstract

One of alternative strategies in prevention and therapy of Benign Prostatic Hyperplasia (BPH) is the usage of a number of traditional herbal plants. In recent years, there is an increasing interest in some members of the Epilobium genus in the treatment of BPH. So far there is no data about molecular mechanisms of action of Epilobium angustifolium L. extracts in liver. The aim of this study was to investigate the influence of the standardized water extract from the Epilobium angustifolium L. on expression level of selected P450 cytochromes encoding genes (CYP3A1 and CYP7A1) in rats liver tissues, which protein products are involved in steroids metabolism. The most abundant fraction of active compounds obtained from the extract was a phenolic fraction (24.36% m/m phenolic compounds and small amounts of flavonoglycosides – 0.91% and sterols – 0.09%). The plant extract showed the strongest and significant inhibitory CYP3A1/2 mRNA expression effect in rat liver. Our results suggest that a standardized E. angustifolium extract can regulate transcription level of investigated CYP genes in a different manner since a strong inhibition of CYP3A1 mRNA expression and slight induction of CYP7A1 may be a result of different extract action on the investigated CYP’s transcription machinery in rat liver cells.
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